酶聯(lián)免疫吸附試驗（ELISA）：ELISA是“酶聯(lián)免疫吸附測定法。”這是一個快速的免疫化學試驗，涉及一種酶（一種蛋白質(zhì)，催化生化反應(yīng)）。它還涉及抗體或抗原（免疫分子）。
酶聯(lián)免疫吸附試驗是用來檢測具有抗原特性的物質(zhì)，主要是蛋白質(zhì)（相對于小分子和離子，如葡萄糖和鉀）。其中包括激素，細菌抗原和抗體。

elisa試劑盒的文獻翻譯

這個試驗有變異，但zui基本的是附在固體表面的抗體。這種抗體的親和力（將鎖上）例如，利益，物質(zhì)，人絨毛膜促性腺激素（HGC），常用的測量表明妊娠蛋白質(zhì)。將純化的絨毛膜促性腺激素（偶聯(lián)）的混合物與酶和測試樣品（血液、尿液等）加入到測試系統(tǒng)中。如果沒有HCG在測試樣品中存在，那么只有絨毛膜促性腺激素與鏈接酶將結(jié)合。在測試樣本中存在的HCG越多，與絨毛膜結(jié)合的絨毛膜促性腺激素結(jié)合的可能性就越小。然后添加酶作用的物質(zhì)，并以某種方式測量產(chǎn)品的量，如溶液顏色的變化。
ELISA檢測通常是高度敏感和特異的與放射免疫法（RIA）試驗比較。他們有優(yōu)勢，不需要放射性同位素和輻射計數(shù)裝置。

elisa試劑盒的文獻翻譯

Enzyme-linked immunosorbent assay (ELISA): ELISA stands for "enzyme-linked immunosorbent assay." This is a rapid immunochemical test that involves an enzyme (a protein that catalyzes a biochemical reaction). It also involves an antibody or antigen (immunologic molecules).

ELISA tests are utilized to detect substances that have antigenic properties, primarily proteins (as opposed to small molecules and ions such as glucose and potassium). Some of these include hormones, bacterial antigens and antibodies.

There are variations of this test, but the most basic consists of an antibody attached to a solid surface. This antibody has affinity for (will latch on to) the substance of interest, for example, human chorionic gonadotropin (HGC), the commonly measured protein which indicates pregnancy. A mixture of purified HCG linked (coupled) to an enzyme and the test sample (blood, urine, etc) are added to the test system. If no HCG is present in the test sample, then only HCG with linked enzyme will bind. The more HCG which is present in the test sample, the less enzyme linked HCG will bind. The substance the enzyme acts on is then added, and the amount of product measured in some way, such as a change in color of the solution.

ELISA tests are generally highly sensitive and specific and compare favorably with radioimmune assay (RIA) tests. They have the added advantages of not needing radioisotopes or a radiation-counting apparatus.

elisa試劑盒的文獻翻譯